Standardization of Protocol for Production of Synthetic Seed of Brahmi (Bacopa monnieri) and Assessment of their Longevity under Differential Storage Condition

Brahmi [Bacopa monnieri (L) Pennel] is an important medicinal small succulent, medicinal herb belongs to the family ‘Scrophulariaceae’ which is commonly known as Jala Brahmi or Nira-Brahmi. National Medicinal Plants Board (NMPB), estimated an annual demand of Bacopa in 2004-2005 was 6621.8 tonnes, with a 7% annual growth rate. Micropropagation techniques and production of synthetic seeds can be a breakthrough to overcome the low availability of Brahmi propagating materials. So, the present study was conducted to standardize the protocol for preparation of synthetic seeds of Brahmi using nodal segment. The experiment was conducted with five treatments by using combinations of different concentrations of sodium alginate and calcium chloride and preparing two types of synthetic seeds (single layer and double layer). Further the longevity of both single layer and double layered synthetic seeds was studied under two different storage temperatures at 25°C (ambient condition) and 4°C (refrigerator) up to 40 days by inoculating them in two different types of media. The result showed no significant effect of concentrations of gelling agent and complexing agent on the germination percentage of synthetic seeds. The number of shoots per synthetic seed varied from 22.00 to 29.35 with 100% germination and maximum shoots/synthetic seed and shoot length was obtained by using T5 (single layered with MS media, 3% sodium alginate and 2.5% calcium chloride). Maximum number of days of storage can be observed in the single layer synthetic seeds when stored at 4°C along with media followed by double layer synthetic seeds when stored at 4°C along with media. However, seeds stored on vermiculite got dried up in the initial days of storage. Germination was found to be higher in seeds stored at 4°C when compared to seeds stored at 25°C. The study as a whole elucidated the opportunity for the use of synthetic seed as an alternative to traditional propagation system for Brahmi.