The Prophylactic Role of Chrysin against Clonazepam Induced Brain Toxicity in Male Albino Rats

Background: The bioactive substance chyrus is found in bee propolis and all plants of the species Passiflora. It is well recognized to have neuroprotective properties and a wide range of pharmacological activity.

Purpose: In this work, the consequence of Chrysin administration (50 mg/kg b.wt/day) on brain toxicity caused by Clonazepam (CZP, 2mg/kg b.wt/day) were investigated by measuring NaK ATPase, neuronal oxidative stress, neuro- inflammation, and DNA fragmentation.

Study Design: In our investigation, we used male albino rats 4 weeks old and weighing 60 ± 5 g. There were four groups of ten rats apiece: Group 1: the control group which treated a vehicle with 1% w/v Tween 80. Group 2: given 1% w/v Tween 80-suspended Clonazepam (CZP) at a dose of 2 mg/kg b.wt./day. In Group 3: Chrysin suspended in 1% w/v Tween 80 was given at a rate of 50 mg/kg b.wt/day. Group 4: Clonazepam (CZP) and Chrysin were given at the same prior dosages as before (2 mg/kg b.wt/day for Clonazepam (CZP) and 50 mg/kg b.wt/day for Chrysin).

Methods: Malondialdehyde, nitric oxide, DNA fragmentation, sodium oxide dismutase, catalase, and Na-K ATPase contents were estimated.

Results: According to the biochemical analysis, after the Clonazepam therapy, the brain's contents of malondialdehyde (MDA), nitric oxide (NO), and DNA fragmentation increased, while those of superoxide dismutase (SOD), catalase (CAT), and NaK ATPase activities declined.

Conversely, the biochemical screening of animal brain tissue administered with CZP+ Chyrsin revealed an improvement in the brain tissue's ability to withstand the damage caused by CZP.