Evaluation of the In-vitro Anti- Inflammatory Activity of Nerium oleander Flower Extract by Using Protein Denaturation Method in India

In this present study anti-inflammatory potential of Nerium Oleander was investigated. Unpleasant side effects associated with prolonged use of current anti-inflammatory drugs have necessitated a need for new drugs with limited side effects. Plants rich in antioxidants have been suggested as potential sources of anti-inflammatory compounds. The intricate biological reaction of vascular tissues to damaging stimuli, such as infections, damaged cells, or irritants, includes inflammation. Its symptoms include redness, swelling, discomfort, stiffness, and loss of joint function. NSAIDs are currently used to treat inflammation. Unfortunately these drugs cause increased risk of blood clot resulting in heart attacks and strokes. Therefore, the developments of potent anti-inflammatory drugs from the natural products are now under considerations. Natural products are rich source for discovery of new drugs because of their chemical diversity. A natural product from medicinal plants plays a major role to cure many diseases associated with inflammation. The conventional drug available in the market to treat inflammation produces various side-effects. Based on the resulted the ethonolic extract of the flowers showed the highest anti inflammatory activity. The increments in absorbance of test samples with respect to control indicated stabilization of protein i.e. inhibition of heat-induced protein (albumin) denaturation by Nerium oleander and reference drug diclofenac sodium. The anti-inflammatory activity was found to be concentration dependent and may be attributed to the presence of phytochemicals content in the flowers of Nerium oleander. Over all, It can be concluded that Nerium oleander possessed marked anti-inflammatory effect against the denaturation of protein.