ANTI-INFLAMMATORY ACTIVITY OF SILVER NANOPARTICLES SYNTHESISED USING Andrographis paniculata AND Phyllanthus niruri

Background: The nanostructured noble metals have gained popularity in several medical applications such as molecular imaging, drug delivery, diagnosis and treatment of various diseases. Silver nanoparticles synthesized using plant extract will be eco-friendly, simple and reliable methods offering therapeutic options which are safe, free of side effects and effective for a wide range of diseases. In our study we have chosen Andrographis paniculata and Phyllanthus niruri which are widely used in Ayurveda for problems of the stomach, GIT and to treat chronic fever.

Aim: The aim of the present study is to analyze the anti-inflammatory activity of Andrographis paniculata and Phyllanthus niruri on plant mediated silver nanoparticles

Materials and Method: Andrographis paniculata and Phyllanthus niruri plant leaves were collected and extract was prepared. 1 Milli molar of silver nitrate was added to 90 ml double distilled water and to this 10 ml of prepared plant extract was added. The synthesized stable silver nanoparticles were characterized by UV- Vis spectroscopy, which showed shift peak at 550 nm. The anti-inflammatory activity of silver nanoparticles synthesized using plant extract was investigated by albumin denaturation assay.

Results: The silver nanoparticles synthesized using the plant extract shows that the maximum inhibition obtained was 62% at 100 µg/ml which is comparatively less than the standard diclofenac sodium.

Conclusion: The silver nanoparticles synthesized using the plant extract acts as a potent anti-inflammatory agent. It can be recommended as a potential anti-inflammatory agent for further in-depth pharmacological studies in vivo and can be used as a replacement to the commercially available drugs.