ISOLATION OF ACTINOMYCETES FROM Vitis vinifera L. and Annona squamosa L. FRUITS AND SCREENING FOR BIOACTIVE COMPOUNDS

Objectives: The current study focus and highlights on isolation of actinomycetes from Vitis vinifera L. and Annona squamosa L. fruits and screening for bioactive compounds being produced by actinomycetes particularly antibacterial activity and amylase inhibition activity and their role in fruit quality.

Methods: The actinomycetes were isolated from the fruit by pretreatment and growing on selective nutrient medium without and without antibiotics. Antimicrobial activity of extracts of isolates screened by antibiotic disc diffusion method and amylase inhibition activity screened using 3, 5-dinitrosalicylic acid by spectroscopic method.

Results: Total 16 actinomycetes were isolated from Vitis vinifera L. and Annona squamosa L. fruits sample by using calcium carbonate, dry heat pretreatment and grown on medium with and without antibiotics. Out of 16, 5 extracts of isolates shown antibacterial activity against Escherichia coli and Pseudomonas aeruginosa. Extracts of isolate G2 and C2 shown 30 and 50 % amylase inhibitory activity respectively.

Conclusion: The current study reveals that actinomycetes are present on the Vitis vinifera L. and Annona squamosa L. fruits, and they produces antibacterial activity which prevent undesired bacterial growth on fruit helps restore fruit quality. We found that isolated actinomycetes were source of bioactive compounds and amylase inhibition activity that help to prevent starch degradation.