CONTROLLING OF Streptococcus mutans AND Lactobacillus IN In-vitro USING HERBAL FORMULATION OF Ocimum sanctum AND Justicia adhatoda MEDIATED SILVER NANOPARTICLES

Introduction: Nanomedicine has tremendous prospects for the improvement of diagnosis and treatment of human diseases. Use of microbes in biosynthesis of nanoparticles is an environmentally acceptable procedure. There are different types of nanoparticles such as gold, silver, alloy and magnetic. Silver nanoparticles are widely recognized for their anti-microbial activity. Tulsi leaves are quite effective in treating common oral infections. Pytochemical agents namely carvacrol and terpene are present in this plant. While Justicia adathoda also known as ‘Vasaka’ is effective in exhibiting antibacterial, anti-inflammatory activity and also anti-fungal and minimal anti-oxidant and cytotoxic properties.

Aim: To control the activity of Streptococcus mutans and Lactobacillusin- vitro using herbal formulation of Justicia adhatoda and Ocimum santum mediated Silver Nanoparticles.

Objective: 1.Toanalysethe antibacterial activity of biosynthesised Ag nanoparticles using Adhatoda and tulsi.

2.To promote the Justicia adhatoda and Ocimum santum mediated Ag nanoparticles biosynthesis over other artificial and chemically formed AgNps.

Materials and Methods: Different extracts of dried leaf of Tulasi and Justicia adhatoda were used as reducing agents for synthesis of cost effective and environmental friendly silver nanoparticles and tested against Streptococcus mutans and Lactobacillus. UV-Visible spectroscopy was used to monitor the quantitative synthesis of silver nanoparticles and the activity showed considerable inhibitory effects against Streptococcus mutans and Lactobacillus.

Results and Conclusion: Zone of inhibition was observed to be maximum for the highest concentration i.e. 150 µl of the extract against both Streptococcus mutans and Lactobacillus. Hence the extract was found to exhibit effective antimicrobial activity. The peak was highest between the wavelengths of 400 nm and 450nm. The AgNPs formed from the combination of both the herbs Justicia adhatoda and Ocimum santum in the present study has shown considerable antibacterial activity due to their potent inhibitory action against the bacterial colonies.