EFFECTS OF 6-BENZYL AMINO PURINE AND NAPHTHALENE ACETIC ACID ON SHOOT AND ROOT INDUCTION IN PURPLE VARIETY Orthosiphon aristatus Blume Miq (INDONESIA TRADITIONAL MEDICINAL PLANTS)

Asian and European countries have used the O. aristatus plant as raw material for traditional medicines and health drinks Purple varieties of O. aristatus have higher levels of sinensetin than other varieties, but the population has decreased.. Efforts to propagate purple varieties of O. aristatus need to be done, one of which is plant tissue culture (in vitro culture). The explants used were leaf shoots and planted on Murashige and Skoog's (MS) media which was added with a combination of growth regulators 6-benzyl amino purine (BAP) + naphthalene acetic acid (NAA); 2,4-dichlorophenoxyacetic acid (2,4-D) + isopentenyl adenine (2ip); 2,4-D + thidiazuron (TDZ). Root induction was carried out on MS medium + concentration variations indole-3-butyric acid (IBA). The results showed good shoot growth in MS medium plus the BAP + NAA combination with a 100% response to shooting growth, while the combination of 2,4-D + 2ip and 2,4-D + TDZ media did not succeed in growing shoots. Optimal root induction was found in MS + IBA media of 0.75 ppm. Media MS + BAP 2 ppm + NAA 3 ppm succeeded in shoots growing purple varieties of O. aristatus well.