ANTIBIOFILM ACTIVITY OF Elaeocarpus ganitrus AGAINST METHICILLIN RESISTANT STAPHYLOCOCCUS SPECIES (MRSS) CAUSING BOVINE MASTITIS

Elaeocarpus ganitrus is commonly known as Rudraksha exhibit a wide range of pharmacological activities which include anti-inflammatory, analgesic, and antimicrobial properties. Medicinal plants traditionally used against bovine mastitis are therapeutically active against bacterial pathogens. This study was aimed to evaluate the antimicrobial potential of E. ganitrus plant seed extracts against biofilm-forming Staphylococcus spp. E. ganitrus plant seed extracts were phytochemically screened for Alkaloids, Flavonoids, glycosides, and Saponins and checked for in vitro antibacterial activity at different concentrations against different Staphylococcus spp by agar well diffusion method. Silver nanoparticles (AgNPs) synthesized from the E. ganitrus were characterized by UV spectroscopy, Fourier Infrared Spectrometry (FTIR), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray analysis (EDAX). E. ganitrus extract showed positive results for flavonoids, tannins, reducing sugars and steroids and the absence of glycosides and alkaloids. UV spectroscopy, SEM, EDAX, and FTIR confirm the presence of AgNPs. The Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of methanol extract and AgNPs against fifteen Methicillin-Resistant Staphylococcus aureus (MRSS) samples were determined and found to significantly inhibit the activity against the tested bacterial strains. The Minimum Inhibitory effect was observed when the microbes were treated with the AgNPs extract (p-value- 0.0001) when compared to the plant seed extract. E. ganitrus and AgNPs extracts were found to be potential against MRSS causing bovine mastitis infection and support the possible use of these phototherapeutic agents in the clinical management of the disease with low cost and fewer side effects.