COMPARATIVE STUDY OF INDOLAMINE 2, 3-DIOXYGENASE EXPRESSION IN ENDOMETRIAL AND BONE MARROW MESENCHYMAL STEM CELLS

Mesenchymal stem cells (MSCs) are considered as ideal cell sources in cell therapy and regenerative medicine for owing the ability of suppressing immune system. Indoleamine 2, 3-dioxygenase (IDO1), the major kynurenine pathway (KP) enzyme, is expressed in MSCs in response to interferon gamma (IFN-γ) and it is considered as the main molecular mechanism of immunosuppression. IDO1 is involved in degradation of tryptophan that is absorbed by lymphocytes as an essential amino acid for growth. This study conducted in comparative form to evaluate the potential role of endometrial mesenchymal stem cells (eMSCs) on immune suppression/modulation between human mesenchymal stem cells of bone marrow and endometrium.
Mesenchymal stem cells from bone marrow and endometrium were plated in culture flask at density of 2 ×105 cells/ml in the presence of 100IU/ml and 500IU/ml INF-γ (IFN-gamma) and IDO1 expression was studied after 72 hours by quantitative RT-PCR (qRT-PCR) and immunocytochemistry.
We found that expression level of IDO1 gene was significantly up-regulated after exposure of human MSCs to IFN-γ and after 72 hours (p<0.05), addition to this we showed that IDO1 expression was higher in endometrial cells than bone marrow cells.