A Validated Bioanalytical Method for Quantification of Ziprasidone in Rabbit Plasma by LC-MS/MS: Application to a Pharmacokinetic Study

A Liquid-liquid extraction method was developed for quantification of Ziprasidone in Rabbit plasma by a suitable bio-analytical method with LC-MS/MS. Ziprasidone d8 was used as an internal standard for Ziprasidone. Hypurity C18, 150 x 4.6 mm, 5 µm column used for chromatographic separation of analyte followed by detection with mass spectrometry. The total run time was 4.0 minutes. The proposed method has been validated with the linear range of 0.05 – 200.00 ng/mL for Ziprasidone. The intra-run and inter-run precision values were within 0.625 to 0.947% and 2.182 to 3.198% for Ziprasidone. The overall recovery for Ziprasidone and Ziprasidone d8 was 92.57% and 95.70% respectively. This validated method was successfully applied into the pharmacokinetic study of Rabbit plasma.